Software to design crosslinks for protein crystal stabilization
| dc.contributor.author | Sebesta, Jacob Christopher, author | |
| dc.contributor.author | Snow, Christopher, advisor | |
| dc.contributor.author | Fisk, Nick, committee member | |
| dc.contributor.author | Rappe, Anthony, committee member | |
| dc.date.accessioned | 2015-08-27T03:57:24Z | |
| dc.date.available | 2017-06-03T03:57:24Z | |
| dc.date.issued | 2015 | |
| dc.description.abstract | Programmable materials allow properties as specific locations in the material to be modified through reliable encoding. One class of such materials are protein crystals that allow changes to be made through genetic manipulation. Protein crystals are well-ordered and highly porous materials, but they are also easily dissolved, limiting their utility. Crosslinking techniques previously developed often have a deleterious effects on the crystal order. In this work, we introduce software to design specific crosslinks across protein crystal interfaces using disulfide and dityrosine crosslinks as well as a variety of small molecule crosslinkers used in protein conjugation. The software is a general tool for specific crosslinking that introduces a number of improvements on previous disulfide design software. Several of the disulfide and dityrosine designs were assembled in the lab and one of the disulfide crosslink designs was confirmed using X-ray diffraction. | |
| dc.format.medium | born digital | |
| dc.format.medium | masters theses | |
| dc.identifier.uri | http://hdl.handle.net/10217/167018 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2000-2019 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.title | Software to design crosslinks for protein crystal stabilization | |
| dc.type | Text | |
| dcterms.embargo.expires | 2017-06-03 | |
| dcterms.embargo.terms | 2017-06-03 | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Chemical and Biological Engineering | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.S.) |
Files
Original bundle
1 - 1 of 1
Loading...
- Name:
- Sebesta_colostate_0053N_12984.pdf
- Size:
- 1004.24 KB
- Format:
- Adobe Portable Document Format