Long chain polyunsaturated fatty acids and oxytocin signaling in pregnant human myometrial smooth muscle cells
| dc.contributor.author | Kim, Paul Y., author | |
| dc.contributor.author | Allen, Kenneth G. D., advisor | |
| dc.date.accessioned | 2024-03-13T19:53:55Z | |
| dc.date.available | 2024-03-13T19:53:55Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Since the suggestion by Olsen and colleagues that the pattern of exceptionally high birth weight in the Faroe Islands is related to a high intake of seafood, a number of epidemiological studies and interventional clinical trials have explored the possibility that consumption of long chain n-3 polyunsaturated fatty acids (LC n-3 PUFA) abundant in fish and fish oil, such as 20:5 eicosapentaenoic acid (EPA) and 22:6 docosahexaenoic acid (DHA), may lengthen gestational duration. A dose response relation between dietary LC n-3 PUFA and gestational duration has emerged wherein expectant women who consume small amounts of fish stand to benefit the most from LC n-3 PUFA supplementation. The mechanisms are not well understood. Although prostaglandins (PG) of the 2-series are known to play a role in the initiation and progress of labor, an interpretation based on modulation of PG biosynthesis appears unlikely since idiopathic preterm labor is accompanied by low PG concentrations. Consequently, we investigated one potential PG-independent mechanism of LC n-3 PUFA action using a pregnant human myometrial smooth muscle cell line, PHM1-41, as a model. Our primary goal was to characterize the effect of DHA treatment on the signaling pathway of oxytocin, a potent uterotonic hormone involved in labor. The addition of 10 µM to 100 µM DHA to the culture media for 48 hrs resulted in incorporation and dose dependent enrichment of DHA in membrane lipid. DHA significantly inhibited IP3 elaboration (at 30 µM and 100 µM) and [Ca2+]i mobilization (at 100 µM) in response to oxytocin stimulation compared with bovine serum albumin (BSA) control and equimolar 18:1 oleic acid (OA). DHA at 30 µM and 100 µM significantly reduced receptor density in the membrane (Bmax) without altering the binding affinity (Kd) or rate of receptor internalization. These findings may be relevant to the reports of dietary fish and fish oil consumption prolonging gestation. | |
| dc.format.medium | born digital | |
| dc.format.medium | doctoral dissertations | |
| dc.identifier | ETDF_Kim_2009_3400977.pdf | |
| dc.identifier.uri | https://hdl.handle.net/10217/237820 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2000-2019 | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.rights.license | Per the terms of a contractual agreement, all use of this item is limited to the non-commercial use of Colorado State University and its authorized users. | |
| dc.subject | myometrium | |
| dc.subject | oxytocin | |
| dc.subject | polyunsaturated fatty acids | |
| dc.subject | smooth muscle cells | |
| dc.subject | molecular biology | |
| dc.subject | cellular biology | |
| dc.subject | nutrition | |
| dc.title | Long chain polyunsaturated fatty acids and oxytocin signaling in pregnant human myometrial smooth muscle cells | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Cell and Molecular Biology | |
| thesis.degree.grantor | Colorado State University | |
| thesis.degree.level | Doctoral | |
| thesis.degree.name | Doctor of Philosophy (Ph.D.) |
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