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Development and characterization of wheat mutants resistant to acetyl co-enzyme A carboxylase inhibitors

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dc.contributor.authorOstlie, Michael H., author
dc.contributor.authorWestra, Philip, advisor
dc.contributor.authorHaley, Scott, committee member
dc.contributor.authorLapitan, Nora, committee member
dc.contributor.authorShaner, Dale, committee member
dc.date.accessioned2007-01-03T08:10:08Z
dc.date.available2007-01-03T08:10:08Z
dc.date.issued2012
dc.description.abstractWeed management in bread wheat (Triticum aestivum L.) is currently limited when undesirable grass species are present due to limited options for herbicidal selectivity between the species. The incorporation of an acetyl co-enzyme A carboxylase (ACCase) inhibitor tolerance trait in wheat would allow growers to expand the arsenal and effectiveness available for managing troublesome grass species. To develop this new trait, ethyl methanesulfonate mutagenesis (EMS) was utilized to induce point mutations in the ACCase gene. Seeds mutagenized with 0.75% EMS for 2.5 hours were grown and seed was collected. Lethal applications of clethodim and quizalofop were applied to plants in the M2 generation. Approximately 200 surviving plants were collected out of the total of 2.5 million M2 seeds planted. M3 greenhouse screening revealed roughly half the plants selected with quizalofop in the M2 generation contained an increased level of resistance to the herbicide. Dose response studies confirmed a two- to- eight fold increase in resistance over wild-type wheat and limited cross-resistance across other ACCase inhibiting herbicides. DNA sequencing of the ACCase gene in quizalofop mutants uncovered a novel point mutation resulting in an Ala - > Val substitution at position 2004 from the Alopecurus myosuroides numbering scheme. A 14C-based enzyme assay established a three- to- tenfold increase in ACCase activity in the presence of quizalofop from plants containing the new mutation. The experiment successfully generated quizalofop resistant mutant wheat and led to the discovery of a previously unknown point mutation. This mutation has the potential for future implementation in a wheat cultivar resistant to quizalofop.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierOstlie_colostate_0053A_10923.pdf
dc.identifierETDF2012400266BSPM
dc.identifier.urihttp://hdl.handle.net/10217/67621
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectACCase
dc.subjectEMS
dc.subjectmutation
dc.subjectquizalofop
dc.subjecttriticum aestivum
dc.subjectwheat
dc.titleDevelopment and characterization of wheat mutants resistant to acetyl co-enzyme A carboxylase inhibitors
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineBioagricultural Sciences and Pest Management
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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