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Primary and secondary metabolism in Centaurea maculosa and their potential roles in invasion biology

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dc.contributor.authorBroeckling, Corey D., author
dc.contributor.authorVivanco, Jorge M., advisor
dc.date.accessioned2024-03-13T18:50:53Z
dc.date.available2024-03-13T18:50:53Z
dc.date.issued2008
dc.description.abstractCentaurea maculosa is plant species native to Eurasia which has become invasive in North America, in part through allelopathic behavior. Allelopathy remains a highly debated subject, and a more firm understanding is necessary. C. maculosa is reported to secrete catechin as an allelochemical that is toxic to North American native plants. In this dissertation, a novel colorimetric assay for use in detection of catechin from soils is described and validated. This assay is highly sensitive, selective, and fast, which should allow for more detailed measures of catechin under field conditions, and ultimately a better understanding of the variation in catechin accumulation. To increase the depth of understanding of catechin biosynthesis, I report the cloning and characterization of C. maculosa dihydroflavonol reductase (CmDFR), a gene very likely to be necessary for catechin biosynthesis. To expand our view of metabolism beyond catechin, metabolome analysis is applied to field collected plant material, and demonstrate that the physiology of C. maculosa varies with the surrounding plant community -- plants growing in patches with high C. maculosa density tend to be accumulate higher levels of secondary metabolites than plants growing in the company of few conspecifics. Finally, the results of a study that clarifies the role of root exudates in structuring the soil fungal community are presented in the context of invasion biology.
dc.description.abstractThis study demonstrates that root exudates are capable of influencing the soil fungal community even in the absence of the plant in a species specific manner, and that the effect of root exudates on the soil fungal community is qualitatively similar to the effect of the whole plant.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierETDF_Broeckling_2008_3346438.pdf
dc.identifier.urihttps://hdl.handle.net/10217/237600
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.rights.licensePer the terms of a contractual agreement, all use of this item is limited to the non-commercial use of Colorado State University and its authorized users.
dc.subjectallelopathy
dc.subjectCentaurea maculosa
dc.subjectinvasive species
dc.subjectsecondary metabolism
dc.subjectsoil microbes
dc.subjectmolecular biology
dc.subjectplant biology
dc.titlePrimary and secondary metabolism in Centaurea maculosa and their potential roles in invasion biology
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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