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M-CSF and GM-CSF influence naïve murine alveolar macrophage differentiation and function in vitro

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dc.contributor.authorHiggins, Jonathan R., author
dc.contributor.authorHiggins, David M., author
dc.contributor.authorLee, Eric J., author
dc.contributor.authorOrme, Ian M., author
dc.contributor.authorGonzalez-Juarrero, Mercedes, author
dc.date.accessioned2015-07-28T16:08:11Z
dc.date.available2015-07-28T16:08:11Z
dc.date.issued2008
dc.descriptionThis research has been published in Relative Levels of M-CSF and GM-CSF Influence the Specific Generation of Macrophage Populations during Infection with Mycobacterium tuberculosis by Higgins, D et al. in Journal of Immunology, 2008, 180: 4892-4900.
dc.description.abstractThe objective of this study was to compare the effect of M-CSF and GM-CSF in their abilities to affect the functional and phenotypical characteristics of AMs. In this study, we harvested naïve uninfected murine alveolar macrophages by bronchoalveolar lavage (BAL) and cultured in the presence of M-CSF, GM-CSF or both. GM-CSF stimulated cell proliferation at a higher rate when measured by relative loss of CFDA-SE dye and had higher phagocytic capacity than M-CSF cultured cells. In contrast, alveolar macrophages cultured in the presence of M-CSF exhibited a dendritic-cell-like morphology and upregulated expression of dendriticcell- associated markers like CCR7, MHC II and DEC205. The dendritic cell character of M-CSF-treated AMs was also manifested in their higher capacity to stimulate CD4+ T cell proliferation in a mixed lymphocyte reaction (MLR). This study demonstrates that M-CSF has a strong ability to differentiate AMs into DC-like cells.
dc.description.awardHighest Honors.
dc.format.mediumStudent works
dc.format.mediumposters
dc.identifier.urihttp://hdl.handle.net/10217/1822
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2008 Projects
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectM-CSF
dc.subjectGM-CSF
dc.subjectalveolar macrophages
dc.subjectAM
dc.subjectdendritic cells
dc.subjectDC
dc.titleM-CSF and GM-CSF influence naïve murine alveolar macrophage differentiation and function in vitro
dc.typeStillImage
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineMicrobiology, Immunology and Pathology
thesis.degree.disciplineVeterinary Medicine and Biomedical Sciences

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