Measurement of microbial biomass N:C by chloroform fumigation-incubation
| dc.contributor.author | Harris, D., author | |
| dc.contributor.author | Voroney, R. P., author | |
| dc.contributor.author | Paul, Eldor A., author | |
| dc.contributor.author | Agricultural Institute of Canada, publisher | |
| dc.date.accessioned | 2007-01-03T06:07:11Z | |
| dc.date.available | 2007-01-03T06:07:11Z | |
| dc.date.issued | 1997-11 | |
| dc.description.abstract | We present a calculation for soil microbial biomass N:C ratio determined from a 10-d incubation following chloroform fumigation. The calculation is based on a mathematical model of the N content of the pre- and post-fumigation soil microbial biomass and the growth yield of the biomass that develops after fumigation. Biomass N is calculated from the N:C ratio and biomass C. The mineralization of bacteria and fungi, with different N contents, added to fumigated soils was used to establish the model parameters. The model was tested against an independent set of measurements and considers two assumptions: 1) The ratio of N:C mineralized from killed biomass is equal to the ratio of N:C mineralized from soil non-biomass constituents. 2) More realistically, the N and C mineralization in the fumigated soil, from sources other than killed biomass, is a residual fraction of the N and C mineralization in the unfumigated soil. Biomass C:N ratios calculated without a control correction (assumption 1) were, on average, 20% wider than corrected values (assumption 2). Biomass N calculated as the product of N:C and biomass C was compared with published values for several data sets. The new calculation method was robust even when net immobilization of N followed fumigation. | |
| dc.format.medium | born digital | |
| dc.format.medium | articles | |
| dc.identifier.bibliographicCitation | Harris, D., R. P. Voroney and E. A. Paul, Measurement of Microbial Biomass N:C by Chloroform Fumigation-Incubation. Canadian Journal of Soil Science 77, no. 4 (November 1997): 507-514. https://dx.doi.org/10.4141/S96-064. | |
| dc.identifier.doi | https://dx.doi.org/10.4141/S96-064 | |
| dc.identifier.uri | http://hdl.handle.net/10217/80890 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | Faculty Publications | |
| dc.rights | ©1997 Agricultural Institute of Canada. | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | soil microbial biomass | |
| dc.subject | C:N ratio | |
| dc.subject | chloroform fumigation | |
| dc.subject | nitrogen | |
| dc.title | Measurement of microbial biomass N:C by chloroform fumigation-incubation | |
| dc.type | Text |
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