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Metabolic engineering of Arabidopsis for butanetriol production using bacterial genes

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dc.contributor.authorAbdel-Ghany, Salah E., author
dc.contributor.authorDay, Irene, author
dc.contributor.authorHeuberger, Adam L., author
dc.contributor.authorBroeckling, Corey D., author
dc.contributor.authorReddy, Anireddy S. N., author
dc.contributor.authorElsevier Inc., publisher
dc.date.accessioned2007-01-03T06:08:38Z
dc.date.available2007-01-03T06:08:38Z
dc.date.issued2013-11
dc.description.abstract1,2,4-butanetriol (butanetriol) is a useful precursor for the synthesis of the energetic material butanetriol trinitrate and several pharmaceutical compounds. Bacterial synthesis of butanetriol from xylose or arabinose takes place in a pathway that requires four enzymes. To produce butanetriol in plants by expressing bacterial enzymes, we cloned native bacterial or codon optimized synthetic genes under different promoters into a binary vector and stably transformed Arabidopsis plants. Transgenic lines expressing introduced genes were analyzed for the production of butanetriol using gas chromatography coupled to mass spectrometry (GC-MS). Soil-grown transgenic plants expressing these genes produced up to 20 µg/g of butanetriol. To test if an exogenous supply of pentose sugar precursors would enhance the butanetriol level, transgenic plants were grown in a medium supplemented with either xylose or arabinose and the amount of butanetriol was quantified. Plants expressing synthetic genes in the arabinose pathway showed up to a forty-fold increase in butanetriol levels after arabinose was added to the medium. Transgenic plants expressing either bacterial or synthetic xylose pathways, or the arabinose pathway showed toxicity symptoms when xylose or arabinose was added to the medium, suggesting that a by-product in the pathway or butanetriol affected plant growth. Furthermore, the metabolite profile of plants expressing arabinose and xylose pathways was altered. Our results demonstrate that bacterial pathways that produce butanetriol can be engineered into plants to produce this chemical. This proof-of-concept study for phytoproduction of butanetriol paves the way to further manipulate metabolic pathways in plants to enhance the level of butanetriol production.
dc.description.sponsorshipPublished with support from the Colorado State University Libraries Open Access Research and Scholarship Fund.
dc.format.mediumborn digital
dc.format.mediumarticles
dc.identifier.bibliographicCitationAbdel-Ghany, Salah E., Irene Day, Adam L. Heuberger, Corey D. Broeckling, and Anireddy S.N. Reddy, Metabolic Engineering of Arabidopsis for Butanetriol Production Using Bacterial Genes. Metabolic Engineering 20 (November 2013): 109-120. http://dx.doi.org/10.1016/j.ymben.2013.10.003
dc.identifier.doihttps://dx.doi.org/10.1016/j.ymben.2013.10.003
dc.identifier.urihttp://hdl.handle.net/10217/80926
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartofOpen Access Research and Scholarship Fund (OARS)
dc.rights.licenseThis article is open access and distributed under the terms and conditions of the Creative Commons Attribution 3.0 Unported (CC BY 3.0).
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/
dc.subjectphytoproduction
dc.subjectarabinose
dc.subjectbutanetriol
dc.subjectBTTN
dc.subject.lcshArabidopsis
dc.subject.lcshXylose
dc.titleMetabolic engineering of Arabidopsis for butanetriol production using bacterial genes
dc.typeText

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