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Protein based technologies to identify, study, and control intracellular processes

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dc.contributor.authorBruce, Virginia Jane, author
dc.contributor.authorMcNaughton, Brian, advisor
dc.contributor.authorRoss, Eric, committee member
dc.contributor.authorDi Pietro, Santiago, committee member
dc.contributor.authorFisk, Nick, committee member
dc.date.accessioned2017-09-14T16:05:08Z
dc.date.available2018-09-12T16:04:38Z
dc.date.issued2017
dc.description.abstractProteins are increasingly used as basic research tools and therapeutics. Their large size, complex structure, and functional group diversity, by virtue of amino acids, often permit recognition of surfaces that challenge small-molecules. Fundamentally, this thesis describes protein based solutions to identifying macromolecules that function inside mammalian cells, enabling visualization and the study of complex biological processes in cellular environments. It also describes the development of engineered polycationic cell-penetrating nanobodies that access the cytosol and thus possibly represent a general solution to intracellularly targeted biologics drug discovery. Collectively, the work described in this thesis reports on: (1) a prostate cancer cell-selective cell penetrating peptide, and its optimization; (2) cell-penetrating nanobodies that access the cytosol of mammalian cells; (3) the use of engineered protein assemblies for bioanalytical reagents and to visualize transcription and translation in mammalian cells, and; (4) a new protein reassembly-based technology to measure cytosolic residence of intracellularly delivered proteins. Technologies and methods described in this work advance the use of proteins in basic science and therapeutically-relevant environments.
dc.format.mediumborn digital
dc.format.mediumdoctoral dissertations
dc.identifierBruce_colostate_0053A_14317.pdf
dc.identifier.urihttps://hdl.handle.net/10217/183950
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectcellular delivery
dc.subjectproteins
dc.subjectcell penetrating peptides
dc.subjectresurfacing
dc.subjectprotein engineering
dc.titleProtein based technologies to identify, study, and control intracellular processes
dc.typeText
dcterms.embargo.expires2018-09-12
dcterms.embargo.terms2018-09-12
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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