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Influence of barley genetics on beer chemistry, flavor, and flavor stability

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dc.contributor.authorBettenhausen, Harmonie M., author
dc.contributor.authorBunning, Marisa, advisor
dc.contributor.authorHeuberger, Adam, advisor
dc.contributor.authorAvens, Jack, committee member
dc.contributor.authorBroeckling, Corey, committee member
dc.contributor.authorStone, Martha, committee member
dc.date.accessioned2018-01-17T16:46:05Z
dc.date.available2019-01-12T16:46:10Z
dc.date.issued2017
dc.description.abstractIn the brewing industry, identifying superior ingredients that provide distinct flavors is an important area of research. While the contribution of raw ingredients such as yeast and hops to flavor is well understood, it is currently unclear if different genotypes of barley provide unique flavor to beer. In brewing, barley is malted to provide saccharides and enzymes for fermentation, however the malt also contains thousands of metabolites that may influence flavor. The goals of this study were to determine (i) if there would be metabolite differences among six commercial barley genotypes, (ii) if differences in barley chemistry are reflected in the chemistry of the beer, (iii) if the differences in the beer chemistry impact sensory attributes of beer, through flavor and flavor stability, and (iv) if there are barley and/or malt metabolites that can be markers for beer flavor and/or flavor stability. Six distinct malts were brewed into six beers using a recipe designed to evaluate differences in flavor. The malts were derived from the barley genotypes: Copeland, Expedition, Full Pint, Meredith, Metcalfe and PolarStar were grown and malted in either Canada or the U.S. Metabolomics was used to characterize chemical variation among the six malts and beers using RP-UHPLC-MS, HILIC-MS (non-volatile metabolites), HS/SPME-GC-MS (volatiles), and ICP-MS (metals). The metabolomics analysis detected 5,042 compounds in malt, and 217 were annotated as known metabolites and included amines (20 metabolites), amino acids (36), fatty acids/lipids (40), sugars (11), phenols (30), and others (80). A total of 4,568 compounds were detected in beer and included 246 annotated metabolites and included amines (9), amino acids (37), fatty acids/lipids/fatty acyls (28), sugars (10), phenols (20), esters (89), aldehydes (21), others (31). The chemical profiles of the six malts and beers were evaluated for metabolite variation using principal component analysis (PCA) and analysis of variance (ANOVA). Principal component analysis was conducted on the annotated metabolites and demonstrated that each of the six malts and beers contained unique chemical profiles. ANOVA characterized 150/217 malt metabolites (69.1%) and 150/246 beer metabolites (60.9%) varied among genotype (ANOVA, FDR adjusted p < 0.05). The six beers were evaluated for flavor using a modified Quantitative Descriptive Analysis® (QDA) for 45 sensory traits at 0, 4, and 8 weeks of storage at 13 °C. PCA characterized flavor differences among the six beers at 8 weeks and Full Pint was described as fruity and Meredith as corn chip. The metabolite and sensory data were integrated using two approaches: Spearman's correlation and two-way orthogonal projection to latent structures (O2PLS). The analyses revealed associations between fruity or corn chip flavor in beer with beer purines/pyrimidines, volatile ketones, amines, and phenolics; and malt lipids, saccharides, phenols, amines, and alkaloids. Taken together, these data support a role of barley metabolites in beer flavor and flavor stability. As a raw ingredient, malted barley genotypes should be evaluated for a contribution to flavor, and this may be a future target for plant breeding efforts to selectively improve flavor and flavor stability quality in beer.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierBettenhausen_colostate_0053N_14565.pdf
dc.identifier.urihttps://hdl.handle.net/10217/185742
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.titleInfluence of barley genetics on beer chemistry, flavor, and flavor stability
dc.typeText
dcterms.embargo.expires2019-01-12
dcterms.embargo.terms2019-01-12
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineFood Science and Human Nutrition
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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