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Nucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length and mRNA export

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dc.contributor.authorSagawa, Fumihiko, author
dc.contributor.authorWilusz, Jeffrey, advisor
dc.contributor.authorWilusz, Carol J., advisor
dc.contributor.authorReddy, Anireddy S. N., committee member
dc.contributor.authorThamm, Douglas, committee member
dc.date.accessioned2007-01-03T05:16:23Z
dc.date.available2007-01-03T05:16:23Z
dc.date.issued2011
dc.description.abstractDuring polyadenylation the multi-functional protein nucleophosmin is deposited onto all cellular mRNAs analyzed. Premature termination of poly(A) tail synthesis using cordycepin abrogates deposition of the protein onto the mRNA, indicating natural termination of poly(A) addition is required for nucleophosmin binding. Nucleophosmin appears to be a bona fide member of the complex involved in 3' end processing as it is directly associated with the AAUAAA-binding CPSF-160 protein and can be co-immunoprecipitated with other polyadenylation factors. Furthermore, reduction in the levels of nucleophosmin results in hyperadenylation of mRNAs, consistent with alterations in poly(A) tail chain termination. Finally, knock down of nucleophosmin results in retention of poly(A)+ RNAs in the cell nucleus, indicating that nucleophosmin binding influences mRNA export. Collectively these data suggest that nucleophosmin plays an important role in poly(A) tail length determination and helps network 3' end processing with other aspects of nuclear mRNA maturation.
dc.format.mediumborn digital
dc.format.mediummasters theses
dc.identifierSagawa_colostate_0053N_10385.pdf
dc.identifier.urihttp://hdl.handle.net/10217/47448
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectmRNA export
dc.subjectNucleophosmin
dc.subjectpolyadenylation
dc.subjectpoly(A) tail
dc.subject.lcshRNA
dc.titleNucleophosmin deposition during mRNA 3' end processing influences poly(A) tail length and mRNA export
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineCell and Molecular Biology
thesis.degree.grantorColorado State University
thesis.degree.levelMasters
thesis.degree.nameMaster of Science (M.S.)

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