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Steady-state and time-resolved spectroscopy to probe the effects of confinement on Cy3 and the dynamics of AOT/iso-octane reverse micelles

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dc.contributor.authorMcPhee, Jeffrey, author
dc.contributor.authorVan Orden, Alan K., advisor
dc.contributor.authorLevinger, Nancy E., committee member
dc.contributor.authorBarisas, B. George, committee member
dc.contributor.authorPrieto, Amy L., committee member
dc.contributor.authorLuger, Karolin, committee member
dc.date.accessioned2007-01-03T04:55:23Z
dc.date.available2007-01-03T04:55:23Z
dc.date.issued2010
dc.description.abstractThis dissertation describes the use of steady-state and time-resolved spectroscopy to probe the effects of localized confinement on the water soluble dye Cyanine-3 (Cy3) and the dynamics of intermicellar interactions using fluorescence correlation spectroscopy (FCS). The first set of experiments presents a wide range of steady-state and time-resolved spectroscopy data which indicate that the Cy3 molecules form H-aggregates at concentrations so dilute (nM) that this behavior is not observed in bulk aqueous solution. This unique behavior allowed for a series of FCS and dynamic light scattering measurements to be performed on the same system. These results indicate the formation of a transient reverse micelle dimer, whose lifetime has been identified to be on the order of 15 μs. Furthermore, preliminary experiments are presented on the same reverse micelle system containing the Rhodamine 6G and the results are consistent with those obtained for Cy3 in the reverse micelles. Lastly, fluorescence resonance energy transfer within the reverse micelles was investigated using Cy3 and Cy5. The preliminary results suggest that FRET may be occurring within this extremely confined environment. The work as a whole provides insight into the nature of confinement as well as the dynamics occurring within the world of reverse micelles.
dc.format.mediumdoctoral dissertations
dc.identifier2010_Fall_McPhee_Jeffrey.pdf
dc.identifierETDF2010300049CHEM
dc.identifier.urihttp://hdl.handle.net/10217/45999
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2000-2019
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subjectFlorescence spectroscopy
dc.subject.lcshSpectrum analysis
dc.subject.lcshReversed micelles
dc.titleSteady-state and time-resolved spectroscopy to probe the effects of confinement on Cy3 and the dynamics of AOT/iso-octane reverse micelles
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineChemistry
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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