Does Flotillin play a role in lipid raft organization of the GnRH receptor and its ability to transduce an intracellular signal?
| dc.contributor.author | Phillips, Adam Paul, author | |
| dc.contributor.author | Mrdutt, Mary Megan, author | |
| dc.contributor.author | Clay, Colin McKeown, author | |
| dc.date.accessioned | 2007-01-03T07:25:52Z | |
| dc.date.available | 2007-01-03T07:25:52Z | |
| dc.date.issued | 2009 | |
| dc.description.abstract | Gonadotropin-Releasing Hormone (GnRH) and its subsequent signaling through the GnRH Receptor (GnRH-R) is critical for gonadal development and control of reproduction function. The GnRH-R is a member of the G-protein coupled receptor (GPCR) superfamily and is localized to specialized low-density areas on the cell membrane termed lipid rafts. These raft domains are implicated in GPCR coupled signaling by spatially organizing receptors and their associated signaling proteins to specific domains in the plasma membranes of cells. These raft domains appear to play an important role in the organization of GnRH-R and the signaling of GnRH to MAPkinase. Flotillin-1 is a protein thought to be intricately involved in the organization of rafts and the trafficking of proteins to raft domains. To examine the potential role of flotillin in GnRH signaling, gonadotrope derived αT3-1 cells were transfected with a specific siRNA for Flotillin-1 with the long-term goal of assessing the impact of Flotillin-1 deficiency on GnRHR trafficking to lipid rafts and signaling to intracellular targets including extracellular signal regulated kinase (ERK). Objectives: to use si-GLO Red to determine the transfection efficiency of Flotillin-1 siRNA; to use siRNA technology to knockdown Flotillin-1 expression; to determine the effect of Flotillin-1 knockdown on GnRH-R signaling. | |
| dc.description.award | Highest Honors. | |
| dc.format.medium | Student works | |
| dc.format.medium | posters | |
| dc.identifier.uri | http://hdl.handle.net/10217/24127 | |
| dc.language | English | |
| dc.language.iso | eng | |
| dc.publisher | Colorado State University. Libraries | |
| dc.relation.ispartof | 2009 Projects | |
| dc.rights | Copyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright. | |
| dc.subject | Flotillin-1 | |
| dc.subject | gonadotropin-releasing hormone | |
| dc.subject | GnRH | |
| dc.subject | GnRH receptor | |
| dc.subject | GnRH-R | |
| dc.subject | siRNA | |
| dc.title | Does Flotillin play a role in lipid raft organization of the GnRH receptor and its ability to transduce an intracellular signal? | |
| dc.type | StillImage | |
| dc.type | Text | |
| dcterms.rights.dpla | This Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s). | |
| thesis.degree.discipline | Veterinary Medicine and Biomedical Sciences | |
| thesis.degree.discipline | Biomedical Sciences |
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