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Endoplasmic reticulum stress increases glucose production in vivo via effects on liver glycogenolysis and glucose-6-phosphatase activity

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dc.contributor.authorGonzales, Jon C., author
dc.contributor.authorWang, Dong, author
dc.contributor.authorWei, Yuren, author
dc.contributor.authorPagliassotti, Michael J., author
dc.date.accessioned2007-01-03T05:51:05Z
dc.date.available2007-01-03T05:51:05Z
dc.date.issued2006
dc.description.abstractRecent evidence suggests that endoplasmic reticulum (ER) stress can induce impairments in both insulin secretion and insulin action. The aim of the present study was to examine the effects of ER stress on glucose production in vivo. Fasted rats were anesthetized and catheters were placed in the carotid artery, jugular vein, and jejunal vein. A pancreatic clamp was performed in which somatostatin was infused to inhibit pancreatic insulin and glucagon secretion. These hormones were then replaced at basal levels. To examine the effects of ER stress on glucose production, 6,6-2H2 Glucose was infused in the absence (CON, n =4) or presence of jejunal vein tunicamycin delivery (TUN, n =6). TUN induces ER stress through inhibition of protein glycosylation. Arterial insulin, glucagon, corticosterone, and free fatty acid concentrations were constant throughout experiments and were not different between groups. Glucose concentration and production increased by 76.2+-24.2 mg/dl and 2.6+-1.2 mg/kg/min (mean+-SDEV), respectively, in TUN, but did not change in CON. Liver glucose-6-phosphatase (G6Pase) and phosphoenopyruvate carboxykinase mRNA were not different between groups. Liver, but not kidney, G6Pase activity (nmoles/mg protein/30min) was increased in TUN (7.2+-2.1) vs. CON (0.2+-0.3). Liver glycogen concentration was reduced by 62% in TUN vs. CON. These data suggest that experimental induction of ER stress can increase the production of glucose in vivo, in part, via activation of hepatic glycogenolysis and G6Pase.
dc.description.awardCollege Honors.
dc.format.mediumStudent works
dc.format.mediumposters
dc.identifier.urihttp://hdl.handle.net/10217/543
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relation.ispartof2006 Projects
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subject.lcshEndoplasmic reticulum
dc.subject.lcshGlucose -- Synthesis
dc.subject.lcshGlucose-6-phosphatase
dc.titleEndoplasmic reticulum stress increases glucose production in vivo via effects on liver glycogenolysis and glucose-6-phosphatase activity
dc.typeStillImage
dc.typeText
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineNatural Sciences
thesis.degree.disciplineBiochemistry and Molecular Biology

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Endoplasmic reticulum stress increases glucose production in vivo via effects on liver glycogenolysis and glucose-6-phosphatase activity
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