Investigation of adipose-derived mesenchymal stem cells interaction with electrospun demineralized bone matrix nanofiber scaffolds
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Nanofiber demineralized bone matrix (DBM) scaffolds were fabricated by electrospinning, and their ability to support cell adhesion and cell viability of murine adipose-derived mesenchymal stem cells (AD-MSCs) for short-term in culture media was investigated. Poly (ε-caprolactone) (PCL) scaffolds were used as control surfaces. Live cell stain calcein-AM and CellTiter 96® Non-Radioactive Cell Proliferation assays were used for cell adhesion and cell proliferation, respectively. DBM scaffolds supported greater cell adhesion compared to PCL nanofiber scaffolds. For cell viability, the two types of ...