A MALDI-TOF mass spectrometry approach to biomarker discovery and validation
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Identifying biochemical markers for disease is a difficult endeavor. It requires methods that can quantify multiple components simultaneously, are high throughput, cost effective and deliver high precision and accuracy. In this thesis I explore the potential of matrix-assisted laser desorption/ionization (MALDI) for these applications. The approach developed for this study is a non-targeted MALDI based method to analyze biological tissues and fluids, and here I demonstrate its utility by way of the analysis of human tear fluid. I hypothesize that biomarker signatures of injury, wound repair ...