The detection of ribonuclease cleavage sites in host and viral RNAs during viral infections
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Ribonucleases are critical components for cellular responses to viruses. RNase L is an endoribonuclease activated during many types of viral infections, yet the RNAs targeted by RNase L to promote its antiviral activities are poorly characterized. To better understand the RNAs cleaved by RNase L during viral infections, I optimized and validated cDNA synthesis and deep sequencing methods that enrich for RNA fragments with terminal 2ˊ, 3ˊ-cyclic phosphates. RNase L, in addition to many other ribonucleases, produces RNA cleavage fragments with terminal 2ˊ, 3ˊ-cyclic phosphates, making 2ˊ, ...