Evaluation of bacterial sampling methods for use with the bacterial tag-encoded flexible (FLX) amplicon pyrosequencing (bTEFAP) technique
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Background: The need to enumerate airborne microorganisms during infectious disease outbreaks, indoor air quality evaluations, and agricultural health studies has identified limitations in culture-based or viable sampling and characterization of bioaerosols. Pyrosequencing promises to be a novel, molecular-based technology that is exceptionally sensitive, low-cost, and provides a reasonable turnaround in the identification, distribution and concentration of aerosolized microorganisms. However, bioaerosol sampling methods for use with pyrosequencing have not been thoroughly evaluated. The intent ...