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Design of a fluorescence technique using double laser pulse excitation for the measurement of molecular Brownian dynamics

Date

1997

Authors

Marconi, Mario Carlo, author
Etcheverry, J. I., author
Torga, J. R., author
Elsevier Science B. V., publisher

Journal Title

Journal ISSN

Volume Title

Abstract

A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors.

Description

Rights Access

Subject

Brownian dynamics
fluorescence anisotropy
rotational depolarization

Citation

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