Preimplantation genetic diagnosis of equine embryos
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In horses, determination of certain genetic traits/alleles in embryos before embryo transfer would be advantageous due to the costs of resulting pregnancies. An attractive option is preimplantation genetic diagnosis (PGD), but to date few biopsied equine embryos have resulted in pregnancies. In the current experiment, 37 embryos ranging from 160 - 575 μm in diameter were biopsied. To obtain embryos, donor mares were monitored using transrectal ultrasonography. When a follicle > 35 mm in diameter was observed, 2,500 IU hCG or 1.5 mg deslorelin acetate was administered, and mares were inseminated ...